Quantitative, Label-Free Live Cell Imaging for the Future of Cell Research
Phase Holographic Imaging (PHI) is a Swedish biotechnology innovator specializing in non-invasive, label-free live cell imaging and analysis. Best known for its flagship product, the HoloMonitor® system, PHI empowers researchers with real time, quantitative insights into live-cell behavior – without the need for stains, dyes, or fluorescence, thus preserving the natural physiology of the cells.
Unlike traditional imaging methods that may induce phototoxicity or stress responses, PHI’s holographic microscopy uses low-intensity laser light and digital holography to continuously capture 3D images of live cells in culture. This allows for long-term observation of cell morphology, movement, growth, and interaction with minimal disruption.
Key features and research applications:
Non-invasive, label-free imaging: Maintains cellular health and native behavior throughout the observation period.
Quantitative data generation: Provides detailed metrics on cell proliferation, motility, morphology, confluency, and death ideal for time lapse studies.
Live cell monitoring: Enables high frequency tracking of individual cells or populations over hours to days.
High-content analysis: Ideal for drug screening, cancer cell behavior, immune response, regenerative medicine, and stem cell research.
The HoloMonitor® M4 system is compact and designed to fit directly inside standard CO₂ incubators, making it highly accessible and compatible with existing laboratory workflows. PHI’s powerful App Suite software automates cell segmentation and data extraction, supporting a range of experiments from basic cell tracking to advanced kinetic analyses.
PHI’s imaging technology is particularly impactful in:
Cancer research: Studying metastasis, invasion, and treatment responses.
Cell therapy and regenerative medicine: Monitoring stem cell growth and differentiation in real-time.
Toxicology and drug discovery: Measuring subtle cellular changes under different compound exposures.
Headquartered in Lund, Sweden, PHI collaborates with leading academic institutions, pharmaceutical companies, and biotechnology firms across the globe. Its innovative approach to live-cell imaging reflects a growing demand for non-invasive, real-time insights into dynamic cell behavior.
Partnering with PHI means gaining access to next-generation cell imaging technology that enhances research accuracy, reduces artifacts, and opens new dimensions in cell biology.